Objective: To establish a high performance capillary electrophoresis(HPCE) method for the determination of hesperidin

glycyrrhizic acid and glycyrrhetinic acid in Erchen Tang. Method: Fused silica capillary coated (50 μm×95 cm) with detection window at 86.5 cm from anode was employed and 50 mmol ·L-1 borax solution (10% acetonitrile

pH 9.28) served as the running buffer. Other conditions were as follows: electrokinetic injection 20 s. analytical voltage 24 kV

detection wavelength 254 nm and temperature 20 ℃. Result: The linearity of hesperidin was in the range of 0.10- 2.40 g ·L-1 (r=0.999 4)

the average recovery was 98.17% and RSD1.49%. The linearity of glycyrrhizic acid was in the range of 0.04- 0.72 g ·L-1 (r=0.999 4)

the average recovery was 98.72% and RSD 2.95%. The linearity of glycyrrhetinic acid was in the range of 0.01- 0.18 g ·L-1(r=0.999 6)

the average recovery was 98.48% and RSD 2.25%. Conclusion: The method is rapid

simple and accurate and it provides the basis of the fingerprints of CE and composite herbal medicines.