HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量

吴怀恩; 劳深; 王雯慧; 韦韬; 黄海燕; 林粒梅

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HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量

更新时间：2024-01-04

- HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量
- Determination of Chlorogenic Acid,Caffeic Acid and Mangiferin in Shiwei Dispensing Granule by HPLC
- 中国实验方剂学杂志 2010年16卷第16期页码：54-57
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- 纸质出版日期：2010
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吴怀恩, 劳深, 王雯慧, 等. HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量[J]. 中国实验方剂学杂志, 2010,16(16):54-57.

WU Huai-en, LAO Shen, WANG Wen-hui, et al. Determination of Chlorogenic Acid,Caffeic Acid and Mangiferin in Shiwei Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 54-57.
吴怀恩, 劳深, 王雯慧, 等. HPLC测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷的含量[J]. 中国实验方剂学杂志, 2010,16(16):54-57. DOI：

WU Huai-en, LAO Shen, WANG Wen-hui, et al. Determination of Chlorogenic Acid,Caffeic Acid and Mangiferin in Shiwei Dispensing Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 54-57. DOI：

摘要

目的 :建立以高效液相色谱法同时测定石韦配方颗粒中绿原酸、咖啡酸及芒果苷含量的方法。方法 :色谱柱为Shim-pack VP-ODS柱(4.6 mm×250 mm

5 μm);流动相为乙腈-0.1%磷酸(9 ∶91);检测波长0~30 min为323 nm

30.1~45min为258 nm;流速为1.0 mL ·min-1。结果 :绿原酸在129.6~302.4 mg ·L-1( r =0.999 8)线性关系良好

平均回收率为98.15%

RSD 1.69%;咖啡酸在13.8~32.2 mg ·L-1( r =0.999 9)线性关系良好

平均回收率为99.93%

RSD 2.98%;芒果苷在151.2~352.8 mg ·L-1( r =0.999 9)线性关系良好

平均回收率为101.43%

RSD 2.00%。结论 :本法简便、快速、重复性好

可用于石韦配方颗粒的质量控制。

Abstract

Objective: To establish an HPLC method for the simultaneously determination of chlorogenic acid

caffeic acid and mangiferin in Shiwei Dispensing Granule. Method: Chlorogenic acid

caffeic acid and mangiferin were separated on Shim-pack VP-ODS (4.6 mm×250 mm

5 μm) column and detected at 323 nm (0~30 min) and 258 nm (30.1~45 min)

with acetonitrile-0.1% phosphoric acid solution (9 ∶91) as mobile phase. The flow rate was 1.0 mL ·min-1. Result: Chlorogenic acid

caffeic acid and mangiferin were linear within the range of 129.6~302.4 mg ·L-1 ( r =0.999 8)

13.8~32.2 mg ·L-1 ( r =0.999 9) and 151.2~352.8 mg ·L-1 ( r =0.999 9) respectively. The average recovery were 98.15% (RSD 1.69%)

99.93% (RSD 2.98%) and 101.43% (RSD 2.00%). Conclusion: The method is simple

reproducible

and it can be used for the quality control of Shiwei Dispensing Granule.

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