CHEN Hong-xi, Zhou Jun, WANG Dian-rong, et al. Determination of Baicalin in Chaihu Jiangan Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 52-54.
CHEN Hong-xi, Zhou Jun, WANG Dian-rong, et al. Determination of Baicalin in Chaihu Jiangan Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 52-54.DOI:
目的 :建立HPLC测定柴胡健肝胶囊中黄芩苷含量的方法。 方法 :色谱柱为Diamonsil C18(4.6 mm×250 mm
5 μm)
流动相甲醇-水-冰醋酸(54 ∶46 ∶0.2)
流速1.5 mL ·min-1
测定波长315 nm。 结果 :黄芩苷线性范围为8.072~48.43 mg ·L-1
平均回收率为99.54%
RSD 0.88%(n=6)。 结论 :本法方便、快速、准确
可用于柴胡健肝胶囊的质量控制。
Abstract
Objective: To establish an HPLC method for determination of baicalin in Chaihu Jiangan Capsules. Method: The chromatogram column was Diamonsil C18(4.6 mm×250 mm
5 μm)
the mobile phase composition was the methanol-water-glacial acetic acid (54 ∶46 ∶0.2) the flow rate was 1.5 mL ·min-1
the detection wavelength was at 315 nm. Result: The linear range of baicalin was 8.072~48.43 g ·L-1
the average recovery was 99.54% with RSD 0.88% (n=6). Conclusion: the method was convenient
quick and accurate.It can be used for quality control of Chaihu Jiangan Capsules.