ObJective: To contrast ingredients of rehmannia dride rhizome with different disct. Method: Carbohydrate was extracted by supersound and water used for solvent

was separated by NH2 chromatographic column and acetonitrile-water(72 ∶28) for mobile phase at 1.0 mL ·min-1

was detected by refraction detector and was assayed by high-performance liquid chromatography. Catalpinoside was extracted by supersound and methanol used for solvent

was separated by C18 chromatographic column and methanol-0.1%phosphoric acid solution at 1.0 mL ·min-1

was detected at λ=210 nm and was assayed by high-performance liquid chromatography. Result :Carbohydrate ingredients of rehmannia dride rhizome and prepared rhizome of rehmannia are conformity

but content are different. Content of catalpinoside have striking difference. Conclusion: The method is good in the aspect of reproducibility and sensitivity.It can be applied to contrast for the ingredients of Rehmannia dride rhizome and prepared rhizome of rehmannia.