ZHOU Nong, GUO Ji-fen, YANG Li-yun, et al. Determination of Diosgenin in var. at Different Collecting Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 54-56.
ZHOU Nong, GUO Ji-fen, YANG Li-yun, et al. Determination of Diosgenin in var. at Different Collecting Time by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 54-56.DOI:
Objective: To determine diosgenin in Paris polyphylla var. yunnanensis at different collecting time. Method: Diosgenin was separated on a Zorbax SB-C18 (4.6 mm×150 mm
5 μm ) column and detected at 203 nm at the flow rate of 1.0 mL ·min- 1 using acetonitrile-water (90 ∶10) as the mobile phase. Column temperature was at 35 ℃. Result : There were differences in the contents of diosgenin in P. polyphylla var. yunnanensis at different collecting time. The contents of diosgenin increased from April to June and reached the highest level in May and June. However
it decreased sharply in June and July. The contents maintained the same level from July to October. Conclusion : The contents of diosgenin in P. polyphylla var. yunnanensis varied with the collecting seasons.