双波长高效液相色谱法测定山茱萸中多种成分的含量

龚卫红; 赵希贤

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双波长高效液相色谱法测定山茱萸中多种成分的含量

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- 双波长高效液相色谱法测定山茱萸中多种成分的含量
- Determination of Double-wavelength Multiple of Content in
- 中国实验方剂学杂志 2010年16卷第14期页码：58-60
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- 纸质出版日期：2010
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龚卫红, 赵希贤. 双波长高效液相色谱法测定山茱萸中多种成分的含量[J]. 中国实验方剂学杂志, 2010,16(14):58-60.

GONG Wei-hong, ZHAO Xi-xian. Determination of Double-wavelength Multiple of Content in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 58-60.
龚卫红, 赵希贤. 双波长高效液相色谱法测定山茱萸中多种成分的含量[J]. 中国实验方剂学杂志, 2010,16(14):58-60. DOI：

GONG Wei-hong, ZHAO Xi-xian. Determination of Double-wavelength Multiple of Content in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 58-60. DOI：

摘要

目的: 建立双波长HPLC测定山茱萸 Cornus officinalis Sieb. et Zucc.中熊果酸、马钱苷含量的方法。方法: 用十八烷基键合硅胶为固定相Shim-pack VP-ODS柱

以乙腈为流动相A

以0.02%磷酸水为流动相B

进行梯度洗脱

检测波长为210

238 nm。结果: 熊果酸在0.1~2.0 μg线性良好(r=0.999 9)。回收率为99.44%(RSD 0.39%

n=6)。马钱苷在0.08~1.6 μg线性良好(r=0.999 9)。回收率为98.92%(RSD0.75%

n=6)。结论: 本方法简便快速

可同时测定山茱萸中多种成分含量

结果准确。

Abstract

Objective: To establish a dual-wavelength HPLC method for the determination of ursolic acid and loganin from Cornus officinalis. Method: Octadecyl bonded silica stationary phase Shim-pack VP-ODS column was used. Gradient elution was performed with acetonitrile as mobile phase A

0.02% phosphoric acid water as mobile phase B. The detection wavelength was set at 210 nm and 238 nm. Result: UA was linear in the range of 0.1- 2.0 μg

(r=0.999 9). The average recovery was 99.44% (RSD 0.39%

n=6). Loganin was linear in the range of 0.08- 1.6 μg. (r=0.999 9). The average recovery was 98.92% (RSD 0.75%

n=6). Conclusion: The method established was simple

rapid and accurate and could be used for simultaneous determination of ursolic acid and loganin from dogwood.

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