Objective: To establish a method for HPLC-fingerprint of Radix Aconiti from different areas. Method: The fingerprint for Radix Aconiti from different areas was established by HPLC. Gemini-NX C18 column was used at 35 ℃ with acetonitrile-0.2% acetic acid (adjusted to pH 10.0 with ammonia water) as mobile phase by gradient eluting

the flow rate was set at 0.8 mL ·min-1

detection wavelength was set at 235 nm and eluting period was 75 min. Result: HPLC-fingerprint of R.aconiti from different areas was established

which contained 12 common peaks

and six peaks in the fingerprint were identified

most of the similarities were over 92%

10 batches of R.aconiti from different areas were classified into three groups based on hierarchical cluster. Conclusion: Fingerprint established was characteristic and specific

combined with the determination it could be used for the quality control of Radix Aconiti .