Objective: To investigate the utilization of grey relational clustering method in evaluating the treatment of Buzhong Yiqi Tang on rats with Qi-deficiency and fever. Method: SD rats were randomly divided into normal group

Qi-deficiency group

Qi-deficiency and fever group

positive control group (aspirin)

high-dose Buzhong Yiqi Tang group

middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group. Swimming strain method and dietary restriction method were utilized to establish the rat model of Qi-deficiency

addition to normal group and Qi-deficiency group

in each group we established the rat model of Qi-deficiency and fever by intraperitoneal injection of LPS. The difference ΔT between rats’ measuring temperature and BBT of each group was then compared. Finally

using gray relational clustering method a comprehensive evaluation of the experimental results was conducted. Result: As for the treatment on rats with Qi-deficiency and fever

aspirin group and high-dose Buzhong Yiqi Tang group were the best

middle-dose Buzhong Yiqi Tang group and low-dose Buzhong Yiqi Tang group ranked behind. Conclusion: Grey relational clustering method can evaluate the effect of Buzhong Yiqi Tang on rats of Qi-deficiency with fever objectively and fairly.