Objective: The essential oil was extracted from the seed of wild Perilla frutescens

and the constituent was analyzed to provide scientific proof for the search of biological activity compositions from P. frutescens to empolder the natural resource. Method : The essential oil from the seed of wild P. frutescens was extracted by steam distillation

and the components of the essential oil were separated and structurally identified by gas chromatography-mass spectrometry. The essential oil was investigated by anti-oxidation activities and antimicrobial activities. Result : Fifty-six components were identified and accounted for 92.77% of the all peak area. The essential oil had anti-oxidation activity and antimicrobial activity. Conclusion: The main chemical constituents of the essential oil were monoterpenoids and sesquiterpenoids compounds. The major components were perillaketone (10.32%)

isoegomaketone (9.73%)

caryophyllene(7.96%)

α-linalool (6.32%)

cinerone (5.28%). The essential oil had obvious anti-oxidation activities for scavenging of ·OH

killed and inhibited the growth of testing bacteria

especially against Staphylococcus aureus CMCC26112 and Candida albicans CMCC850216.