Objective: To select a simple

stable and satisfied experimental model of renal calcium oxalate calculus in rats. Method: After several renal calcium oxalate calculus models currently used (Model group I: administered intragastrically with 1% ethylene glycol free drink+2% ammonium chloride 2 mL/rat per day; model Group Ⅱ: administered intragastrically with 1.25% ethylene glycol+1% ammonium chloride 20 mL ·kg-1 per day; model group Ⅲ: drank 10 mL 1.25% ethylene glycol+1% ammonium chloride and 30 mL water per day; model group Ⅳ: drank 1% ethylene glycol+1% ammonium chloride freely.) experimental rat models were developed. Several biochemical indicators of urine

blood and nephridial tissue were measured and renal pathological specimens were observed. Then the modeling results were comprehensive evaluated. Result: The 24 h urinary BUN

Cr

UA

IP

Ca2+

OX of model groupⅠwere singnificantly higher than the normal control group (groupⅤ) (P<0.01); blood BUN

Cr

UA

IP

Ca2+ were significantly higher than the normal control group (P<0.01); nephridial tissue Ca2+ and OX were significantly higher than the normal control group (P<0.01); and calcium oxalate crystals were distributed in nephridial tissue. The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats renal function breakdown

and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadnt been seen in nephridial tissue. All rats of model group Ⅳ were dead. Conclusion: Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.