LI Xin, CHENG Lan, LI Xue-tao. Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 50-53.
LI Xin, CHENG Lan, LI Xue-tao. Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 50-53.DOI:
Objective: To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes. Method: The HPLC analysis was carried out on a ELITE C18 (4.6 mm×250 mm
5 μm) column with mobile phase consisted of acetonitrile- 0.06 mol ·L-1 KDP buffer (38 ∶62) at a flow rate of 1.0 mL ·min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination. Result: The 1inear range of vinorelbine was 2.08~20.8 μg (r=0.999 9).The average recovery was 98.64% (RSD 1.09%). The average entrapment efficiency was 86.83%. Conclusion: This method is simple
precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.