Objective: To screen the optimal dose of Xiaoyaosan in rats and to improve the anti-depression clinical effect. Method: In this research

two experiments were processed using the model of chronic unpredictable mild stress (CUMS). Amitriptyline and fluoxetine hydrochloride

venlafaxine hydrochloride were used as positive control

respectively. Behavioral changes

such as the weight

open-field

and sugar consumption

were measured. The urine was collected and analyzed by GC-MS

and the result was analyzed with SPSS and SIMCA-P. Result: The results of behavior changes and GC-MS suggested that the control group and model group were significantly different

which indicated that the model was successful. Comparing the treated group with the positive control group and the control group

the dose of 46.3 g ·kg-1 was closest to the control group

there was no significant difference. Conclusion: Xiaoyaosan has the obvious anti-depression effect

and 46.3 g·kg-1 was proved the optimal dose.