Objective: To optimize the best β-cyclodextrin including technology conditions of volatile oil from Pericarpium Citri Reticulatae Viride by orthogonal design. Method: The saturated aqueous solution-ultrasound method was used. Result ：The optimum preparation conditions were established as follows：the proportion of volatile oil to β-cyclodextrin was 1∶8(mL ·g-1），The concentration of alcohol was 90%，The ultrasonic time was 30 min，The water temperature was at 40 ℃～45 ℃. Conclusion ：The different usage of β-cyclodextrin is the principal factor that influence the inclusion effect of volatile oil from Pericarpium Citri Reticulatae Viride，the method of this technology is simple，and the technological process is short，it is suitable for commercial process.