ObJective: Observing the protective effect of Zhenwu Decoction on renal interstitial fibrosis in rat

to explore its role of anti-renal fibrosis and Ⅰ

Ⅲ collagen fibers affected in renal fibrosis. Method :The rat model of chronic renal failure was used. Niaoduqing control group was treated by Niaoduqing solution

Zhenwu Decoction group was treated by Zhenwu Decoction concentrated solution

up to 28 days. After 4 weeks

blood samples were taken from the femoral artery. Serum creatinine

blood urea nitrogen

serum calcium and phosphorus were detected. Renal pathological staining for paraffin sections was carried out by hemotoxylin and eosin staining

periodic acid-silver methenamine (PASM) staining

Masson staining

Sirius red staining

Mallory staining. HE staining was randomly selected for semi-quantitative analysis in tubulointerstitial lesions. Periodic acid-silver methenamine staining

Masson staining

Sirius red staining

Mallory staining were randomly selected for kidney pathology analysis. Result :In PASM mesangial integral optical density quantitative model of mesangial proliferative

Masson staining fibrosis in the area of renal interstitial

Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be better than model group and Niaoduqing control group (P<0.05). Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be less than model group in renal interstitial of type Ⅰ and Ⅲ distribution area (P<0.05). Conclusion :Zhenwu Decoction shows effect of anti-renal interstitial fibrosis. And it inhibit Ⅰ

Ⅱ collagen fibers proliferation

which may be the mechanism for Zhenwu Decoction to treat renal interstitial fibrosis and chronic renal failure.