HPLC测定五味子油软胶囊中五味子醇甲和五味子乙素

郭戎; 单鸣秋; 于生; 张丽; 赵龙

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HPLC测定五味子油软胶囊中五味子醇甲和五味子乙素

更新时间：2024-01-04

- HPLC测定五味子油软胶囊中五味子醇甲和五味子乙素
- Determination of Schisandrin and Schisandrin B In Wuweiziyou Soft Capsule By HPLC
- 中国实验方剂学杂志 2010年16卷第17期页码：72-74
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- 纸质出版日期：2010
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郭戎, 单鸣秋, 于生, 等. HPLC测定五味子油软胶囊中五味子醇甲和五味子乙素[J]. 中国实验方剂学杂志, 2010,16(17):72-74.

GUO Rong, SHAN Ming-qiu, YU Sheng, et al. Determination of Schisandrin and Schisandrin B In Wuweiziyou Soft Capsule By HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 72-74.
郭戎, 单鸣秋, 于生, 等. HPLC测定五味子油软胶囊中五味子醇甲和五味子乙素[J]. 中国实验方剂学杂志, 2010,16(17):72-74. DOI：

GUO Rong, SHAN Ming-qiu, YU Sheng, et al. Determination of Schisandrin and Schisandrin B In Wuweiziyou Soft Capsule By HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 72-74. DOI：

摘要

目的: 建立五味子油软胶囊中五味子醇甲和五味子乙素含量测定方法。方法: 采用高效液相色谱法(HPLC)

色谱柱为Kromasil C18柱(4.6 mm×200 mm

5 μm);流动相水(A)-甲醇(B)梯度洗脱(0~10 min

67% B; 10~15 min

67% ~78% B; 15~35 min

78% B; 35~38 min

78%~67% B;38~43 min

67%B);流速1.0 mL ·min-1;检测波长为254 nm;柱温30 ℃。结果: 五味子醇甲和五味子乙素分别在98.80~989.0 μg (r=0.999 8

n=6)和38.8~388.0 μg (r=0.999 8

n=6)线性关系良好;平均加样回收率(n=6)分别为102.0%和101.0%。结论: 该方法稳定性好、精密度高、重复性强

可用于测定五味子油软胶囊中的五味子醇甲和五味子乙素含量。

Abstract

ObJective: To establish a method for the determination of schisandrin and schisandrin B in Wuweiziyou soft capsule. Method: An HPLC method was set up with Kromasil C18 (4.6 mm×200 mm

5 μm) column. The Mobile phase was H2O (A) and MeOH (B). Gradient elution was performed with 67% B from 0-10 min

67%-78% B from 10-15 min

78% B from 15-35 min

and 78%-67% B from 35-38 min

67% B from 38-43 min. The flow rate was 1.0 mL ·min-1

the column temperature was at 30 ℃ and detection wave length was at 254 nm. Result: The linear ranges of schisandrin and schisandrin B were within 98.80-989.0 g (r=0.999 8

n=6) and 38.8-388.0 g (r=0.999 8

n=6)

and the average recoveries were 102.05% and 100.98% respectively. Conclusion: This method was easy

sensitive

specific and accurate for the determination of schisandrin and schisandrin B in Wuweiziyou soft capsule.

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