LI Xue-ling, LIU Li, ZHONG Reng, et al. HPLC Determination of Gallic Acid and Quercetin in Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 95-97.
LI Xue-ling, LIU Li, ZHONG Reng, et al. HPLC Determination of Gallic Acid and Quercetin in Different Parts of [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 95-97.DOI:
Objective: To determine gallic acid and quercetin from different parts of Archidendron clypearia
and to provide evidence for the rational development and utilization of A.clypearia. Method: HPLC method was used with Kromosil C18 column (4.6 mm×250 mm
5 μm)
and the mobile phases were acetonitrile and 0.2% phosphoric acid solution with the gradient elution. The flow rate was 1 mL ·min-1 and the detection wavelength was 254 nm. Result: Both of the content of gallic acid and quercetin are highest in the leaves
lower in the new shoot and lowest in the shoot
even quercetin cannot be tested in the shoot. Conclusion: The method can be used to control the quality of Archidendron clypearia. It is simple
accurate
reproducible and suitable for simultaneous determination of two active constituents in Archidendron clypearia.