Objective: To investigate the preparation of the novel liposome composed of hydrogenated soybean phosphatidylcholine (HSPC) and soybean phosphatidylcholine (SPC) containing zedoary turmeric oil (ZTO). Method: HPLC analysis and spectrophotometry analysis were used to determine the contents of curcumol and ZTO in ZTO liposomes both composed of HSPC and SPC respectively. The ZTO novel liposomes were prepared by ethanol injection method. The best prescription of ZTO liposome both composed of HSPC and SPC was determined through orthogonal design

using the drug content of liposomes as the evaluation index. Result : When ZTO novel liposome was prepare with the best prescription

both the entrapment efficiency stability and the drug content were high. Conclusion : The method was accurate and applicable. The preparation was feasible and the quality of the novel liposome was stable and controllable.