Objective: To explore the effect of Shugan Lidan (SGLD) therapy for discharging lead and antagonizing the damage of hippocampus. Method: Lead poisoned model of rat was established by giving lead acetate ig for 5 weeks. All rats were randomly divided into seven groups:SGLD high-does group

SGLD middle-does group

SGLD low-does group

Zhiqi Granule group

EDTA-CaNa2group

plumbum dyed model group and normal control group. We measured the concentrations of lead

calcium

zinc in blood by inductively coupled plasma atomic emission spectrometry (ICP); observed morphological changes in hippocampus by electronic microscope; and measured the concentrations of NO

TAOC in the hippocampus cortex by UV spectrophotometry. Result: ① The treatments with SGLD

Zhiqi and EDTA-CaNa2 lowered the level of blood lead significantly

without significant differences among the three groups. Meanwhile the treatments caused the level of calcium and zinc in blood to drop

among the groups medium dose and high dose of SGLD dropped the level of calcium as much as Zhiqi

and the effect was lower than that of EDTA-CaNa2.However

blood zinc was observably dropped less by SGLD than that of Zhiqi and EDTA-CaNa2.② The hippocampus of lead-poisoned rat was injured obviously

but the damage in SGLD groups was the lightest. ③ NO

TAOC in hippocampus of rat was explicitly lack in plumbum dyed model group. The high dose of SGLD could significantly increase the NO

TAOC in hippocampus. And It is better than those in the middle and low doses of SGLD

Zhiqi and EDTA-CaNa2. Conclusion: Shugan Lidan therapy can accelerate the elimination of lead

avoid loss of the endogenous essential elements calcium

zinc

meanwhile antagonize the damage of hippocampus

increase the level of NO

TAOC

and improve learning and memory ability.