Objective: To investigate growth feature of human tubular epithelial cells(HK-2) through cell growth curve. Method: The HK-2 cells were cultured by DMEM / F12(1 ∶1) with 10 % fetal bovine serum.The cells morphology was observed through the microscope

and cells were counted per 24 hours.After observing 7 days the cell growth curve was drawed based on day and amount. Result: We found that HK-2 cells crawling like stone road on the bottle surface.The cell amount began increased on the second day

but the speed was slower during the first day to the third day.The amount increased rapidly from the third day to the sixth day

and the cells were in the exponential phase of growth.On the sixth day

the cells were in saturation density(100%).However

the amount decreased rapidly on the seventh day. Conclusion: The cells were in the incubation period from the first day to the third day

and in the exponential phase of growth from the third day to the sixth day

and in the decline phase on the seventh day.