Objective: The HPLC fingerprints of chemical composition were established to evaluate different collecting time and different growing areas of overground part of Desmodium styracifolium. Method: HPLC analysis was performed on a DiamonsilTM C18 column(4.6 mm×250 mm

5 μm) with the mobile phase in isocratic elution : ethanol(A)-H2O(0.2% phosphoric acid) (28 ∶72). The column temperature was setup at 40 ℃ and the flow-rate was 1.0 mL·min-1. The detection of wavelength was at 271 nm. Result: The D.styracifolium from Yangchun has distinct differences with other place of origin; The accumulation of flavones constituents showed a regular pattern in different collecting time. Conclusoin: The method is reliable and can be helpful on the quality control of D. styracifolium.