Objective: To study the therapeutic effects of the skullcap

Bupleurum chinense and their compatible extract on cell infected by Coxsackievirus B3m (CVB3m) in vitro. Method : We used microcell culture method to observe the effect of the skullcap extract

the B. chinense extract and their compatible extract on antivirus and cell protection. We detected the restrained indexes of the three kinds of drugs on CVB3m and the cell protection rate by the three kinds of drugs when the cell was infected by CVB3m. Result : The skullcap extract could directly kill CVB3m in vitro when its concentration was 1.563 g ·L-1

but the B. chinense extract and their compatible extract did not show this effect. The three kinds of drugs could protect cell infected by CVB3m.The effect of the B. group and the compatibility group was better than the skullcap groups when the drugs were administered in advance(P＜0.05). The effect of the skullcap group and the compatibility group were better than the B. chinense group when the drugs were administered after infection (P＜0.05). Conclusion : The three kinds of drugs can inhibit replication of CVB3m and protect cells from CVB3m. The effect of inhibiting virus of the Skullcap extract was better than the others. The effect of protecting cell of the B. chinense extract and the compatibility group were better than the Skullcap group.