Objective: To set up a method for the determination of 8-epideoxyloganic acid in Duyiwei soft capsule. Method: The quantitative analysis was performed on Diamond C18(4.6 mm×250 mm

59 μm)column and a mixture of methonal-0.025 mol ·L-1 phosphate solution (50 ∶50) was used as the mobile phrase at a flow rate of 1.0 mL·min-1. The column temperature was at 40 ℃ and the detective wavelength was at 238 nm. Result: The linear range was 1.979 2~9.896 μg for 8-epideoxyloganic acid. The average recovery was 96.84% with RSD 2.07%. Conclusion: The method was accurate

reproducible

stable

and can be used as a quantitative standard for determination of iridoid glycosides in Duyiwei soft capsule.