NIE Yang, LI Yuan-xin, LIU Yan, et al. Determination of Tanshinone Ⅱ in Danxiong Dropping Pills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 62-63.
NIE Yang, LI Yuan-xin, LIU Yan, et al. Determination of Tanshinone Ⅱ in Danxiong Dropping Pills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 62-63.DOI:
Objective: To establish the HPLC method for determining tanshinone ⅡA in danxiong dropping pills. Method: The analysis was carried out on a column of Eclipse XDB-C18 (4.6 mm×150 mm
5 μm) with a mobile phase of methanol-water (88 ∶12) at the flow rate of 1.0 mL ·min-1. The detection wavelength was set at 268 nm. Result: Tanshinone ⅡA was linear in range of 4.48~89.6 μg ·mL-1. The average recovery was 99.76% and RSD was 1.45%. Conclusion : The method can be used to determine tanshinone ⅡA in danxiong dropping pills.