Objective: To establish a pre-column derivatization method for HPLC fingerprint analysis of Cornu Cervi Pantotrichum. Method: Cornu Cervi Pantotrichum was derivated by 2

4-dinitrofluorobenzene. HPLC analysis was performed on Column Phenomenex Luna 5u C18 (2) 100R (4.6 mm×250 mm

5 μm)

with a mixture of methanol and acetate buffer as mobile phase in gradient mode. The flow rate was 1.0 mL ·min-1

wavelength was set at 360 nm

and column temperature was at 20 ℃. Result : Seventeen kinds of amino acids in Cornu Cervi Pantotrichum were detected

of which 10 kinds of essential amino acids showed a concordance with the content and market price. Conclusion : Good precision

stability and repetition were showed.The method provides ideas and guide in the quality control of the valuable Cornu Cervi Pantotrichum.