LC-MS/MS法测定独一味中木犀草素

解军波; 张彦青; 吴国娇; 王丽娟

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LC-MS/MS法测定独一味中木犀草素

更新时间：2024-01-04

- LC-MS/MS法测定独一味中木犀草素
- Determination of Luteolin in by Liquid Chromatography Tandem Mass Spectrometry Method
- 中国实验方剂学杂志 2010年16卷第7期页码：64-66
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- 纸质出版日期：2010
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解军波, 张彦青, 吴国娇, 等. LC-MS/MS法测定独一味中木犀草素[J]. 中国实验方剂学杂志, 2010,16(7):64-66.

XIE Jun-bo, ZHANG Yan-qing, WU Guo-jiao, et al. Determination of Luteolin in by Liquid Chromatography Tandem Mass Spectrometry Method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 64-66.
解军波, 张彦青, 吴国娇, 等. LC-MS/MS法测定独一味中木犀草素[J]. 中国实验方剂学杂志, 2010,16(7):64-66. DOI：

XIE Jun-bo, ZHANG Yan-qing, WU Guo-jiao, et al. Determination of Luteolin in by Liquid Chromatography Tandem Mass Spectrometry Method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 64-66. DOI：

摘要

目的 :建立LC-MS/MS检测独一味中木犀草素的含量。方法 :应用超声提取和Agilent G6410B Triple Quad LC/MS检测。Agilent Eclipse Plus C18

(2.1 mm×100 mm

3.5 μm)分析柱;流动相甲醇-0.1%醋酸水( 80 ∶20);流速0.2 mL ·min-1;柱温25 ℃;进样量为20 μL。以液相色谱分离、电喷雾离子化串联质谱进行检测。结果 :木犀草素在5~160 ng ·mL-1线性关系良好

独一味药材中木犀草素的平均加样回收率为99.27%。结论 :该方法快速简便、精密度好、灵敏度高

可用于独一味药材中木犀草素的含量测定。

Abstract

Objective : To establish a rapid resolution liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of luteolin in Herba Lamiophlomis . Method :A LC-MS/MS method was set up using Eclipse Plus C18 (2.1 mm×100 mm

3.5 μm)

methanol- 0.1% acetic acid (80 ∶20) as the mobile phase with a flow rate of 0.2 mL·min-1. Result :The luteolin was linear in the range of 5 to 160 ng ·mL-1and the average recovery was 99.27% for luteolin. Conclusion : The method was simple

rapid and accurate and could be used for the determination of luteolin in Herba Lamiophlomis .

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