Objective: This experiment aim to observe the relationship between sedative and hypnic actions of the Ursolic acid and quercitrin with nitric oxide (NO) and immune regulation mechanism of sleep. Method: The experiment respectively surveyed the indicatrix after intraperitoneal injection of ursolic acid and quercitrin in brain of both nomal and sleep-deprived rats. The assaying of NO is through the method of Griess. The assaying of LI-α and TNF-β is through the method of radioimmunity. Result: ① Two hours after intraperitoneal injection of ursolic acid in normal rat

the level of NO in every encephalic region and IL-1β in blood serum

hippocampus and hypothalamus were enhanced

as well as the amount of TNF-α was slightly increased. But compared with blank group

all results wasn’t different significant. ②Two hours after intraperitoneal injection of quercitrin in normal rat

the amount of NO in every encephalic region was cut down;The levels of LI-1β and TNF-α in blood serum and each encephalic was raised

especially the amount of LI-1β in hypothalamus was increade markedly compared with blank group. ③ Four hours after intraperitoneal injection of ursolic acid in sleep-deprivated rat

the amount of NO in brain were increased compared with either blank or model group

having significant different;the amount of IL-1β in blood serum was significantly elevated compared with either blank or model group. The levels of LI-1β and TNF-α in blood serum

hippocampus and hypothalamus were enhanced

but it was insignificant. ④ Four hours after intraperitoneal injection of quercitrin in sleep-deprived rats

the level of NO in every encephalic region were enhanced

compared with blank and model group

it had significant difference. The level of IL-1β in blood serum was enhanced compared with blank and model group

it had significant different. The amount of LI-1β in hippocampus and hypothalamus was increased significantly compared with blank group. The level of TNF-α in hypothalamus was enhanced significantly. Conclusion: The sedative and hypnic actions of ursolic acid and quercitrin are related with NO and immune regulation mechanism of sleep. Quercitrin of influencing sleep-deprived rat’ sleep is probably related to NO and immunoregulation mechanism. For nomal rat immunoregulation mechanism may be participate in.