Objective: To establish the internal standard multi-control method and applied to the determination of flavonoid compounds in rutaceae plant: citrus aurantium

bitter orange Immature

walnut peel and tangerine peel. Method: Naringin as index

establishing the relative correction factor of naringin

hesperidin and neohesperidin and investigating the durability. Internal standard multi-control method was used to determine the content of flavonoid compounds in rutaceae plant

using included angle cosine method to compare the calculated values with measured values by external standard method. Result: The flow rate

column temperature

columns and instruments did not have significant effect on the relative correction factor and there was no significant difference between the calculated values of determination of flavonoid compounds in rutaceae plant by "internal standard multi-control method" and measured values by external standard method. Conclusion: The established internal standard multi-control method can be used to quantitative analysis and quality control of flavonoid compounds in citrus aurantium

bitter orange immature

walnut peel and tangerine peel.