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纸质出版日期:2010
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王晶娟, 张贵君, 李奇豫. 全蝎蛋白药效组分的生物鉴定法研究[J]. 中国实验方剂学杂志, 2010,16(8):94-95.
WANG Jing-juan, ZHANG Gui-jun, LI Qi-yu. Study the Bioassay Method of Informational Active Components Alignment of Scorpion[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 94-95.
目的 :研究全蝎蛋白药效组分的提取方法
摸索全蝎蛋白药效组分SDS-PAGE的最佳电泳条件
测定全蝎蛋白药效组分电泳图谱条带的大致分子质量
确定其SDS-PAGE的表达特征
从而建立全蝎蛋白药效组分的电泳鉴别法。 方法 :采用水提与盐析法获得全蝎蛋白药效组分
考马斯亮蓝法测定蛋白药效组分的蛋白质含量
SDS-PAGE法和Alpha Ease FC 4.0.0软件测定全蝎蛋白药效组分的蛋白质相对分子质量。 结果 :全蝎蛋白药效组分的蛋白质质量分数为50.37%;得到较好的全蝎蛋白药效组分的10条电泳谱带
相对分子质量分别为94.162 0
66.400 0
40.357 0
27.541 0
22.518 0
19.520 0
12.766 0
11.190 0
9.665 3
8.534 2 KDa。 结论 :这10条谱带是全蝎蛋白药效组分的特征谱带
可用于全蝎蛋白药效组分的鉴别。
Objective: We studied the extracted method of informational active components alignment of scorpion
groped the best electrophoresis conditions
determined the molecular mass of informational active components alignment
assured the expressing characteristic of the electrophoretic bands
and set up the bioassay method of informational active components alignment of Scorpion. Method: We extracted scorpion by water and salt fractionation to obtain informational active components alignment
Coomassie brilliant blue method determined its protein content
and SDS-PAGE electrophoretic technique and Alpha Ease FC 4.0.0 software determined its protein molecular weight. Result: The protein content of informational active components alignment was 50.37%. There were ten bands in the informational active components alignment. Ten bands in the SDS-PAGE were 94.162 0
66.400 0
40.357 0
27.541 0
22.518 0
19.520 0
12.766 0
11.190 0
9.665 3
8.534 2 KD. Conclusion: These ten bands were the characteristic bands of informational active components alignment
and might be used to identify the informational active components alignment.
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