Objective: To analyze the chemical constitutes and effective components of Fufang Fuling Tang (FFFLT)

a traditional Chinese medicine shown to be effective in treating eczema. Method: A rapid method incorporating of ultra fast liquid chromatography-photodiode array detector-electrospray ionization-mass spectra (UFLC-DAD-ESI-MS) profiling was developed for screening the chemical constitutes in FFFLT. Based on the different peaks from FFFLT samples

four herb groups(Ⅰ-Ⅳ) were established. Herb group I was composed of Poria cocos

Paeonia lactiflora Pall

Gardenia jasminoides

Glycyrrhiza uralensis

Alisma orientalis

Phellodendron amurense

Angelica sinensis and Spirodela polyrrhiza; group Ⅱ was composed of P. lactiflora Pall

G. jasminoides

G. uralensis; group Ⅲ was composed of P. cocos and A. sinensis; and group Ⅳ was composed of P. cocos

P. lactiflora Pall

G. jasminoides

G. uralensis and A. sinensis. Sixty mice with allergic contact dermatitis (ACD) induced by DNFB were randomly divided into 6 groups

including Ⅰ-Ⅳ herb treatment groups(HTG)

positive control group (hydrocortisone treatment group

HCTG) and negative control group (physiological saline treatment group

PSTG). The thickness and weight of mouse ears

the pathological changes in ear tissue and the serum levels of interferon (IFN)-γ

interleukin (IL)-4 and IL-10 were observed. Result: 11 chemicals were analyzed and the most abundant constitutes from FFFLT were genipingentiobioside

geniposide

paeoniflorin and liquiritin. Compared with PSTG

significant decrease in ear swelling and dermal inflammatory infiltration were seen in Ⅰ-Ⅳ HTG and the HCTG (P<0.05).ⅠHTG

ⅣHTG and HCTG showed better effects (P<0.01). Conclusion: The pharmacodynamics of FFFLT in treating eczema mostly come from Paeonia lactiflora Pall

Gardenia jasminoides

Glycyrrhiza uralensis

Poria cocos and Angelica sinensis.