Objective: The chromatographic fingerprint of Polygonum Cuspidate was established and the content of polydatin

resveratrol and emodin was measured

which created a new method for evaluating and controlling the quality of Polygonum Cuspidate. Method :Using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (Version2004A) recommended by State Food & Drug Administration

10 different original samples were analyzed and the chromatographic fingerprint of Polygonum cuspidate was constructed with 13 common fingerprint peaks

among which three main fingerprint peaks of polydatin

resveratrol and emodin were identified by reversed-phase high performance liquid chromatography (RP-HPLC). Result :With the simple sample treating technique

the established fingerprint of Polygonum Cuspidate was stable and repeatable

which could be used to distinguish the superior polygonum cuspidate from the inferior polygonum cuspidate from different places and provide an important tool for further quality control of Polygonum Cuspidate.