Objective: To bulid the method for determination of baicalin

jasminoidin and paeonol in Qindan Particles by HPLC. Method : An Aigilent ZORBAX SB-C18 column (4.6 mm×150 mm

5 μm) was used. The mobile phase of baicalin was CH3OH-H2O-P (47 ∶53 ∶0.2)

the detection wave lenghth was set at 280 nm. And the mobile phase of jasminoidin was acetonitrile-H2O (15 ∶85)

the detection wave lenghth was set at 238 nm. For paeonol

the mobile phase was CH3OH-H2O (45 ∶55)

the detection wave lenghth was set at 274 nm. All flow rates were 1.0 mL ·min-1. Result : Under each condition

the calibration curves of baicalin

jasminoidin and paeonol were linear at the ranges of 0.006~0.096 mg ·L-1

0.005~0.080 mg ·L-1 and 0.005~0.050 mg · mL-1

respectively. And the each average recoveries of the method were 98.8% for baicalin (RSD 0.75%

n=9); 99.7% for jasminoidin (RSD 0.94%

n=9); 98.4% for paeonol (RSD 1.41%

n=9). Conclusion : The developed method is simple

accurate and repeatable for determination of baicalin

jasminoidin and paeonol in Qin-dan Particles.