Objective: To investigate the anti-angiogenic mechanism of QHF (a formula of Chinese medicine ingredients

abbreviation Q for Qingrejiedu

H for Huoxuehuayu and F for Fuzhengguben) combined with small dose chemotherapy drug cisplatin(DDP)in H22 mice of hepatocellular carcinoma. Method: The forty eight Balb/c mice were inoculated with cell suspension of mouse hepatocellular carcinoma cell line H22 under the right axillary skin to establish the solid tumor model. Then they were randomly divided into four groups as follows: QHF group

DDP group

QHF+DDP group and NS (normal saline) group. The inhibition rates of tumor growth in tumor-bearing mice by drugs as an indicator were observed. The expression of VEGF

EGFR and MMP-2 in the tissue of mice tumor was detected with immunohistochemisty. Result: The weight of tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.01)

while the weight of mice tumor was significantly lower in QHF+DDP group than that of in QHF group. Their inhibition rate was 47.45%

63.11%

72.95%

respectively. The expression of VEGF and MMP-2 in the tissue of mice tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.05

P<0.01 and P<0.01). Moreover

The expression of EGFR in the tissue of mice tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.01

P<0.05 and P<0.01). However

the difference between QHF+DDP group and other groups has no significance(P>0.05). Conclusion: Both of QHF and small dose DDP have the anti-tumor and anti-angiogenic effect in H22 mice of hepatocellular carcinoma. Moreover

one of anti-angiogenic mechanism may be concerned with the downregulation of the expression of VEGF

EGFR and MMP-2 in the tissue of mice tumor.