荷叶中生物碱提取及纯化工艺研究

朱晓月; 刘虹; 郝彧; 王萌; 张德芹

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荷叶中生物碱提取及纯化工艺研究

更新时间：2024-01-04

- 荷叶中生物碱提取及纯化工艺研究
- Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis
- 中国实验方剂学杂志 2010年16卷第9期页码：22-24
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重大科技专项"重大新药创制"(2009ZX09102-128),治疗糖尿病组分中药的联合开发(2009DFA31070)
- DOI：
  中图分类号：
- 纸质出版日期：2010
- 稿件说明：
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朱晓月, 刘虹, 郝彧, 等. 荷叶中生物碱提取及纯化工艺研究[J]. 中国实验方剂学杂志, 2010,16(9):22-24.

ZHU Xiao-yue, Liu Hong, HAO Yu, et al. Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 22-24.
朱晓月, 刘虹, 郝彧, 等. 荷叶中生物碱提取及纯化工艺研究[J]. 中国实验方剂学杂志, 2010,16(9):22-24. DOI：

ZHU Xiao-yue, Liu Hong, HAO Yu, et al. Technology Study on Extraction and Purification of Total Alkaloids from Folium Nelumbinis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 22-24. DOI：

摘要

目的 :优选荷叶总生物碱的提取纯化工艺。方法 :荷叶药材经过冷浸、浓缩、索氏提取、萃取、碱析

分离荷叶总生物碱

并以酸性染料比色法测定荷叶总生物碱含量。结果 :荷叶用含0.1%盐酸的70%乙醇浸泡提取36 h

提取3次后中和、浓缩

再以乙酸乙酯为溶媒索氏提取3 h

再以pH 1的酸水及乙酸乙酯先后进行萃取

所得乙酸乙酯萃取液用40%氢氧化钠溶液碱析

所制荷叶总生物碱质量分数达到90%以上。结论 :此提取纯化工艺操作简便

方法可行

重复性好

为荷叶总生物碱的开发利用提供了实验依据。

Abstract

Objective: To study the extraction and purification technology of total alkaloids in Folium Nelumbinis. Method: The total alkaloids were obtained by impregnation

concentration

Soxhlet extraction and alkaline separation. Acid dye colorimetry was used to determine total alkaloids in Folium Nelumbinis. Result: Folium Nelumbinis was soaked in 1% HCl/70% ethanol for 36 h 3 times. After neutralization and concentration

Soxhlet extraction was carried out for 3 hours with ethyl acetate. Then the concentrate was extracted successively by acidic water at pH 1 and ethyl acetate. 40% NaOH was used for alkaline separation. The content of total alkaloids was above 90%. Conclusion: This extraction and purification technology was convenient

feasible

and reproducible

that would provide basic for the research and development of Folium Nelumbinis.

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