Objective: To determine the best extraction process of effective part of Prunus tomentosa Thunb by modern scientific methods. Method: Orthogonal design was used to screen and optimize the extraction conditions of P. tomentosa

and animal model of frostbite were used to observe the effect of the extractions. Result: The best extraction condition was: 8 times 95% ethanol was added and extracted 2 times (1.5 h/time) by Rope extractor. The extraction could significantly reduce the inflammation of swelling and prevent blisters and tissue necrosis at the rabbit’s ears. The extract had the significant therapeutic effect for the mild to moderate frostbite of rabbit’s ear. Conclusion: The optimized extraction method was established and the extract had moderate effect of anti-inflammation and anti-frostbite. The results also provide the scientific data for further studying on the new traditional medicine.