Objective: To discuss effects of prescriptions for esophageal carcinoma in different concentrations on the proliferation of esophageal carcinoma cell line EC9706 stimulated by hEGF; to discuss inhibition mechanism of the prescriptions on esophageal carcinoma EC9706 cells stimulated by hEGF from the view of growth signal transduction. Methods: Human esophageal carcinoma cell line EC9706 cultured in vitro treated with hEGF and Qigesan(Q)

Shashenmaidongtang(S)

Tongyoutang(T) and Buqiyunpitang(B)

cells proliferation is assayed by MTT

cells proliferation and cells cycle are measured by FCM. Associated proteins within growth signal pathway mediated by PLC-γ1 were determined by western blot. Results: The half maximum inhibition rates(IC50) of Q

S and T are 849

1 004

1 615 μg·mL. These 3 prescrip- tions could inhibit associated proteins expression within signal pathway mediated by PLC-γ1

the effects of T and Q were better than S. Conclusion: Q S and T could inhibit cells proliferation stimulated by hEGF. The inhibition of B is very weak. The prescriptions influence EC9706 cells proliferation by inhibiting proteins expression associated with growth signal pathway mediated by PLC-γ1.