Objective: To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology

structure

function and protein of mitochondria. Method : Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups

including a control group

DM model group

DM+ WeichangshuⅠgroup

DM+ Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential

respiratory chain and mitochondrial protein were detected using flow cytometry

fluorescence labeling and Western Blot respectively. Result :Weichangshu could ameliorate the mitochondrial morphology

structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential

ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion :Weichangshu could promoted the gastrointestinal motility

which might be related with the regulation of mitochondrial kinetic energy.