Objective: To study the effect of traditional Chinese medicine Yigan Zhuanyin San (YGZYS) on HBsAg and HBeAg of HepG2.2.15 cell. Method: The TC50 and TC0 of YGZYS to the HepG2.2.15 cell were determined by MTT assay. At non-toxic range of concentration

five different concentrations of YGZYS were added respectively to HepG2.2.15 cells. At 4d and 8d after treatment with drugs

the supernatant of HepG2.2.15 were collected for determining the levels of HBsAg and HBeAg by ELISA. Result: TC50 5.386 g ·L-1

TC0 0.736 g ·L-1. YGZYS had little cellulotoxicity. YGZYS at the largest non-cellulotoxic concentration could suppress HBsAg and HBeAg expression in HepG2.2.15 cell

and the therapeutic index (TI)>2

YGZYS appeared to be an effective and low toxic anti-HBV drug. Conclusion: YGZYS can suppress HBsAg and HBeAg expression in HepG2.2.15 cell

and is a lower toxic drug.