Objective: To investigate the protective effect and the mechanism of Herbal Lophatheri flavonoids (HLF) on mice loaded with restraint stress. Method: The filtrate of extraction of Herbal Lophatheri was adsorbed by Hp20 macroporous exchange resin

eluted by ethanol. After oral administration of HLF 125

250 and 500 mg·kg-1 for five days. The mice were restrained for 18 h after 30 min of the last intragastric administration to induce liver injury. The protective effects were evaluated by assessing alanine aminotransferase (ALT) levels in plasma. The contents of NO and malondialdehyde (MDA) in liver were performed respectively. Oxygen radical absorbance capacity (ORAC) assay was used to measure the antioxidant capacity. Results : Compared with the stress model

HLF could decrease activities of ALT in the plasma and the levels of MDA and the contents of NO in liver. And it could significantly increase ORAC activity in the plasma and liver. Conclusion : Herbal Lophatheri flavonoids exert protective effect on liver injury in mice loaded with restraint stress.