Objective: To determine the optimum extraction process of total flavonoids from dewaxed propolis with ethanol by warm-immersion. Method: Removed bee wax with water from raw propolis before extraction. With total flavonoids yield as index

based on the single factors test

orthogonal experiment was performed so as to optimize the extraction parameters. Result: The optimum extraction process of total flavonoids from dewaxed propolis with ethanol by warm-immersion was as follows: cold leaching for 1 h

ethanol concentration 80%

solid-liquid ratio 1 ∶12

extracting for 1 h

extracting temperature at 60 ℃

extracting for 2 times. Conclusion: The optimum extraction process was achieved

which is stable

feasible

energy-saving

short production cycle

high extraction efficiency

suitable for industrialization.