ZHANG Fang-xiang, LIN Zhao-xia. HPLC for quantification of glycyrrhizic acid, 6-gingerol, honokiol and magnolol in Wenfei Zhike Capsule[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 83-85.
ZHANG Fang-xiang, LIN Zhao-xia. HPLC for quantification of glycyrrhizic acid, 6-gingerol, honokiol and magnolol in Wenfei Zhike Capsule[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(7): 83-85.DOI:
Objective: To establish HPLC method for the Determination of glycyrrhizic acid
6-gingerol
honokiol and magnolol in Wenfei Zhike Capsule. Method: LUNA ODS C18 BDS(4.6 mm×200 mm
5 μm)was used with methanol-0.05 mol·L-1 ammonium acetate-glacial acetic acid (65 ∶35 ∶0.5)as a mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was 250 nm. The column temperature was 30℃. Result: The average recovery of was 98.17%
99.14%
98.31% and 99.49% for glycyrrhizic acid
6-gingerol
honokiol and magnolol respectively. Conclusion: The method can be used for quality control of Wenfei Zhike Capsule.