WANG Pei-pei, XU Du-juan, XIA Quan. The extraction and determination the contents of astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 27-30.
WANG Pei-pei, XU Du-juan, XIA Quan. The extraction and determination the contents of astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 27-30.DOI:
Objective: Study on extracting technology and assay of astragaloside in Astragalus. Method :70% ethanol circumfluence extraction was used.Total astragaloside was enriched and purified by extraction refine through water saturated of n-butanol and D101 mcroporous adsorption resin.Different components of astragaloside were isolated by silica gel laminar analysis.Identification by using TLC method and determination the contents by using HPLC-RID Method. Result :Six compounds were isolated from total astragaloside(AS-Ⅰ~AS-Ⅵ)
and 29.4% astragaloside Ⅱ was identified in As-Ⅰ part. AS-Ⅲ which content is 22.82% is mainly contained AstragalosideⅣ by identification.The rest four compounds are required to be identified. Conclusion: The method can be used for astragaloside
HPLC-RID method can be used to determine the content of astragaloside.