Objective: To study content variation of anthraquinones in Rhubarb before and after compatibilities with liquorice root

baikal skullcap root

red peony root

Chinese angelica

Chinese goldthread rhizome

costusroot and gardenia. Method: An HPLC method was established to determine the contents of free and glycoside-combined anthraquinones namely aloe-emodin

rhein

emodin

chrysophanol and physcion in rhubarb before and after compatibility. Result: The total anthraquinones were decreased after compatibility

especially when it was compatible with Chinese goldthread rhizome. Besides

the content of various anthraquinones was changed regularly after compatibility. Conclusion: The content of the five different anthraquinones was influenced by different compatibilities

which may contribute to decrease the toxicity of rhubarb.The study provides the basis when rhubarb is used in clinic.