Objective: To investigate the application of isolated heart perfusion technique to evaluate the bioactivity of antianginal Chinese medicine. Method: Isolated guinea pig heart perfusion was performed with Langendorff method to measure the change in perfusion volume of coronary pre and after administrating the 5 antianginal chinese medicines such as Danshen Injection

Guanxinnin Injection

Xiangdan Injection

Danhong Injection and Danshen for Injection. Result : All 5 injections above could dilate the coronary and increase the perfusion volume

but there was discrepancy in the increase of perfusion volume at the same dose of different lot number from same injection. Conclusion : It may be possible that Langendorff isolated heart perfusion technique would become a good method for evaluating the bioactivity of antianginal Chinese medicine and deserves further probe.