HPLC法同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸

李婉晴; 范俊婷; 刘勇; 樊华

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HPLC法同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸

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- HPLC法同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸
- Determination of Tetramethylpyrazine and Ferulic Acid in Kangxuanyun Granules by RP-HPLC
- 中国实验方剂学杂志 2010年16卷第6期页码：59-61
- 作者机构：
- 作者简介：
- 基金信息：
  
  天津市卫生局中西医结合资助(07018)
- DOI：
  中图分类号：
- 纸质出版日期：2010
- 稿件说明：
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李婉晴, 范俊婷, 刘勇, 等. HPLC法同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸[J]. 中国实验方剂学杂志, 2010,16(6):59-61.

LI Wan-qing, FAN Jun-ting, LIU Yong, et al. Determination of Tetramethylpyrazine and Ferulic Acid in Kangxuanyun Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 59-61.
李婉晴, 范俊婷, 刘勇, 等. HPLC法同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸[J]. 中国实验方剂学杂志, 2010,16(6):59-61. DOI：

LI Wan-qing, FAN Jun-ting, LIU Yong, et al. Determination of Tetramethylpyrazine and Ferulic Acid in Kangxuanyun Granules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 59-61. DOI：

摘要

目的: 建立高效液相色谱法(HPLC)同时测定抗眩晕颗粒中盐酸川芎嗪和阿魏酸的含量。方法: 色谱柱为AGT C18(4.6 mm×250 mm

5 μm);流动相为甲醇-乙腈-1%冰乙酸(1 ∶1 ∶5);检测波长280 nm;流速0.8 mL ·min-1;柱温25 ℃。结果: 盐酸川芎嗪在0.002 4~6.900 0 μg线性良好

r=0.999 8;阿魏酸在2.040 0~58.650 0 μg线性良好

r=0.999 0;盐酸川芎嗪平均回收率98.75%

RSD 1.12%;阿魏酸平均回收率98.45%

RSD 0.62%。结论: 高效液相法可靠、准确

专属性强

重现性好

可用于该制剂质量控制。

Abstract

Objective: To determine the contents of tetramethylpyrazine and ferulic acid in Kangxuanyun Granule. Method: C18 Column was used with methanol acetonitrile for chromatography-1%acetic acid glacial(1 ∶1 ∶5) at a flow rate of 0.8 mL ·min-1. The detecting wavelength was set at 280 nm. Result: The content of ferulic acid and tetramethylpyrazine can be determined by HPLC. The linearity of tetramethylpyrazine was good in the range of 0.002 4~6.900 0 μg

r=0.999 8; The linearity of ferulic acid was good in the range of 2.040 0~58.650 0 μg

r=0.999 0. The average recovery of tetramethylpyrazine was 98.75%

RSD 1.12%

the average recovery of ferulic acid was 98.45%

RSD 0.63%. Conclusion: The method is simple reliable accurate and can be applied as the quantity control method of Kangxuanyun Granule.

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