Determination of Rutin in Motherwort Herb and Herbs with Same Action by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(10): 6-7.
Determination of Rutin in Motherwort Herb and Herbs with Same Action by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(10): 6-7.DOI:
Objective:To develop an HPLC method for the determination of rutin in Motherwort Herb and herbs with the same action.Methods: Analyses were carried out on an Kromasil C18(4.6 mm×150 mm
5 μm)column at 30℃ eluted with a mobile phase consisting of methanol-0.5%phosphoric acid(33∶67)and detected at 254 nm.The flow rate was 1.0 mL·min-1.Results: The linear range of rutin was 0.075~0.375 μg·mL-1.The average recovery was 96.62%(RSD=0.99%).Conclusion: The method is simple and accurate
and can be used for the quality control of Motherwort herb and the herbs with the same action.