Objective:To observe the effect of active component injection of Slauia miltiorrhiza Bge. and Carthamus tinctorius L. (salvianolic acid B and hydroxysafflor yellow A) on myocardial ischemical reperfusion injury in rats. Methods:Myocardial ischemical reperfusion injury model of SD rat was established by ligation of left anterior descending branch for 40 min

and then reperfusion for 120 min. Ten minutes later after coronary ligation

the active component was injected intravenously. The rats were divided into 6 groups (n=10

each group)

they were model group

sham operation group

Danhong injection group

low dose

moderate dose and large dose groups of active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L..The degree of myocardial infarction in rats was determined for every group after 2 h of reperfusion

the contents of cTnT and CK-MB were detected. ST-T change in ECG

TXB2

6-Keto-PGF1α and platelet aggregation in blood plasma were investigated.Results:Compared with model group

every group of active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L. can significantly lower the increase of cTnT and CK-MB in rats(P<0.01)

moderate dose and large dose groups can remarkably lower the maximum platelet aggregation and TXB2 in rats (P<0.01

P<0.01)

correct the unbalance of 6-Keto-PGF1α /TXB2

lower the ST-T extent of ECG in 30 min of reperfusion. Conclusion:Active component injection of Slauia miltiorrhiza Bge.and Carthamus tinctorius L.can lower platelet aggregation induced by reperfusion injury in rats

reduce the leakage of impaired myocardial creatase

inhibit the production of thromboxan

prevent the formation of thrombus

improve the myocardial microcirculation and ischemia.