Objective: To set up a proper technology of refining of the aqueous extract of Wuzhuyu-tang. Method: 7 kind components which had been improved to be absorbable pharmacological ingredients were chosen as index for refining. The static adsorption

dynamic adsorption and leakage curve experiments were done to choose a proper resin. By means of single-factor experiment and orthogonal experiment

the optimum refining conditions were found

such as adsorption time

proportion of sample weight and resin volume

proportion of diameter and height

concentration of decoction and eluent

flow rate

eluent volume and so on. Result: The AB-8 resin had the best separating efficiency when the content in the aqueous extract was 0.33g per 1mL equivalent to raw material

the ratio of dia-height was 1:4

and the volume of 60% ethanol as eluant 8BV with desorption-power 0.1BV/min. Conclusion: The content of 7 kind components were over 70% after refining

5 of them were over 90%. It is concluded that the optimized extracting and refining technology is reasonable and feasible.