Objective:To develop a platform of incubation of plasma in vitro for analysis of conponents of traditional Chinese medicine. Methods:The aconitic alkaloids were as one of the examples. Aconitine

mesaconitine and hypaconitine were added to rabbit plasma and incubated for various durations. The plasma samples were processed with acetonitrile to denature and deposit the protein and extracted the three alkaloids

and the acetonitrile solution was analyzed by a RP chromatographic system. Results: The concentration of aconitine

mesaconitine and hypaconitine in plasma was descreased with the prolonged incubation time

the hydrolysis rate of mesaconitine was the fastest

aconitine was the second and hypaconitine was the third. Conclusion: A simple

stable and controllable method has been established

which has some reference value for pharmacokinetic study of aconitic alkaloids.