Objective:To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin. Methods:HPLC method was used to analyze the content of total glycosides

the adsorption and desorption performance of D101

D201

D301

D401

AB-8

NKA-9 were investigated

and the purification of conditions were inspected. Results:The best macroporous resin for purified extractive of total glycosides in tree peony bark was D101

the concentration of Paeoniflorin was 1.83 mg·mL-1 with pH 6.5

and with the elution flow rate of 1 BV·h-1. Purification of the conditions were as follows:10 BV water and 4 BV 70% ethanol were used as eluant of paeoniflorin

respectively. Portions of 70% ethanol were collected

concentrated

and dried. Conclusion:D101 resin can be used to refine the total glycosides in the water extracts of tree peony bark.