Objective:To analyze the effect on HPLC fingerprint of Flos Genkwa which samples prepared by macroporous resin. Method: The fingerprints of 17 batches of Flos Genkwa were determined. The samples were extracted with methanol

then solvent were distilled off underreduced pressure. The residue were solved by water. Macroporous resin column chromatography was used for separating the water solution. Three parts of eluates

20%

50% and 95% ethanol eluate were obtained

separately. Then they were analyzed by high performance liquid chromatography. Chromatographic conditions: chromatographic column was Diamonsil? C18 (4.6 mm × 250 mm

5 μm)

mobile phase was methanol-water in gradient elute

flow rate was 1 mL·min-1

column temperature was 35 ℃

detective wavelength was set at 332 nm

record time was 40 min. Results: 7 common peaks were obtained in methanol extract

while 11 common peaks were displayed after prepared by maceoporous resin. Conclusion: It can show more information in HPLC fingerprint which samples prepared by macroporous resin. This is a useful method in sample preparation.